26 across
Old
district,
not
one
part
of
America
(6)
Old district, not one part of America (6)
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The definition is at the end of the clue.
How the clue works
The definition is part of America, a US state. For the wordplay, start with O for "old", then add REGION, the "district", and drop the I ("not one"), leaving REGON. Stick the O in front and you've got your state.