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Spirited,
transforming
hotel
into
a
place
of
culture
(5,4)
Spirited, transforming hotel into a place of culture (5,4)
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The definition is at the end of the clue.
How the clue works
Spot a place of culture at the end — that's your definition, a petri dish being where microbial cultures grow. For the wordplay, take "Spirited" and add H (the NATO/radio code for hotel), then "transforming" tells you to shuffle those letters into an anagram, giving PETRI DISH.