24 across
The
precise
hour
business
is
over,
close
up
(6)
The precise hour business is over, close up (6)
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The definition is at the beginning of the clue.
How the clue works
The definition is The precise hour, meaning o'clock. "Business" gives CO which is turned round ("is over") to make OC, followed by LOCK ("close up"), giving OCLOCK.