9 across
Agitate
live,
dumped
freshwater
fish
(5)
Agitate live, dumped freshwater fish (5)
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The definition is at the end of the clue.
How the clue works
Look at freshwater fish at the end — that's what you're after. Agitate gives you RUFFLE, and then live — as in the abbreviation L for a live wire — gets dumped, so you drop that L from RUFFLE and you're left with RUFFE.