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Drink
served
up
by
host
on
vacation
after
dark
(5)
Drink served up by host on vacation after dark (5)
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The definition is at the end of the clue.
How the clue works
The definition is after dark, meaning NIGHT. The wordplay has two parts: first, GIN (drink) is reversed — indicated by "served up" — giving NIG. Then "host on vacation" means we take HOST and remove the inner letters (it's "on vacation", i.e. emptied out), leaving just H and T. Put them together: NIG + HT = NIGHT.